KBK031F

Active

Enzyme Technology

7.5

Course given jointly for second and third cycle

7716 (Biotechnology)

 -02-09

Mats Ohlin

English

Every autumn semester

The aim of the course is to give advanced knowledge about the technical use of enzymes and the possibilities to change and improve enzyme performance for adaptation to technical applications

The theoretical part of the course describes how the catalysts of the biological world, the enzymes, can be used in a variety of contexts. Examples are the use in bioanalysis, refining of food, washing powder, textile treatment and transformation of chemicals.

The general properties of enzymes are well known from previous courses. In this course is described how enzyme molecules can be modified in order to get improved properties for technical use. Examples include genetic and chemical modification and immobilization. Immobilization, i.e. the coupling of enzymes to carrier material, is treated thoroughly. Likewise are the kinetic properties of immobilized enzymes and ways to quantify these properties emphasized.

In the cell the enzymes function in an aqueous environment. Under certain circumstances enzymes can also work efficiently in a non-aqueous environment or in an environment with a low water activity. This opens up for unique possibilities for an enzymatic synthesis of a number of substances, including chiral substances. This kind of use of enzymes in non-conventional media is treated in great detail.

The laboratory practical has two parts. One part where basic techniques concerning immobilization of enzymes are investigated and one research related part where untested experiments with enzymes in organic solvents are designed and carried out.

be able to describe and evaluate methods for modifying enzymes intended for technical use
be able to describe and evaluate methods for enzyme immobilization and for characterization of the properties of immobilized enzymes
describe and evaluate methods for the use of enzymes in non-conventional media
describe the technical use of enzymes

practically carry out enzyme immobilization, characterization of the properties of immobilized enzymes and using enzymes in organic solvents
design, carry out and evaluate enzymatic transformations
make written reports from laboratory practicals in which the experiments and results are described and commented upon and where deviations from expected results are analyzed and discussed

in a group forum orally discuss scientific reports from the literature
analyze, interpret and critically evaluate scientific reports related to enzyme technology

Lectures

Laboratory exercises

Exercises

Written exam

passed reports from the laboratory practicals and passed literature discussion

Failed, pass

KBK011 Biokemi

Undergraduate students have priority for the practical part of the course

P., Danielsson, B., Larsson, P.-O., Månsson, M.-O., Ramanathan & K.: Enzyme Technology.

Course coordinator: Dr Cedric Dicko, cedric.dicko@tbiokem.lth.se; Professor Patrick Adlercreutz, Patrick.Adlercreutz@biotek.lu.se

KBK031F

 -02-09

Mats Ohlin