KBT001F

Temporary

Enzyme Kinetics

7.5

Third-cycle course

7716 (Biotechnology)

 -03-04

FN2/Eva Nordberg Karlsson

English

If sufficient demand

The aim of the course is to give knowledge concerning how rates of enzymatic reactions can be measured and how such measurements can be used to calculate relevant kinetic constants.

Important topics in the course include: mechanisms of enzymatic reactions, measurement of kinetic constants, derivation of rate equations, enzyme inhibition and effects of pH and temperature on enzymatic reactions. Exercises from the course book will be done individually and discussed in the group. After this basic part, the students will select published scientific papers concerning enzyme kinetics, which they will present for the rest of the group. Finally, each student will work on a small project within the enzyme kinetics area on a topic chosen by the student and the course leader. It can be a literature survey, a set of calculations, etc. The results of the project should be summarized briefly in a written report.

• Have knowledge on experimental methodology to measure rates and study the kinetics of enzymatic reactions.
• Have knowledge on mechanisms of enzymatic reactions involving one or two substrates.
• Have knowledge on mechanisms for inhibition of enzymatic reactions.
• Have basic knowledge on derivation of rate equations for enzymatic reactions using King-Altman’s method.
• Have knowledge on how pH and temperature influence the rate of enzymatic reactions.

• Be able to set up an experimental plan for the determination of kinetic constants of enzymatic reactions involving one or two substrates.
• Be able to determine kinetic constants from experimental data for enzymatic reactions involving one or two substrates.
• Be able to set up an experimental plan for the determination of the type of inhibition and values of inhibition constants of enzymatic reactions.
• Be able to use experimental data to determine the type of inhibition and values of inhibition constants for enzymatic reactions.
• Be able to give an oral presentation of a scientific study in the enzyme kinetics area.

• Be able to in a group orally discuss scientific articles concerning enzyme kinetics.
• Be able to analyse and interpret enzyme kinetics data and put it together to a written report.

Seminars

Exercises

Project

Written report

Written assignments

Seminars given by participants

Failed, pass

Basic knowledge of mathematics and biochemistry at university level

Cornish-Bowden, A.: Fundamentals of Enzyme Kinetics. Fourth edition. Wiley-Blackwell, 2012. ISBN 9783527330744.

Given for 8 persons or more.

KBT001F

 -03-04

FN2/Eva Nordberg Karlsson