Gäller från och med: Autumn 2017
Beslutad av: Margareta Sandahl
Datum för fastställande: 2017-05-15
Avdelning: Biophysical Chemistry
Kurstyp: Gemensam kurs, avancerad nivå och forskarnivå
Kursen ges även på avancerad nivå med kurskod: KFKN10
Undervisningsspråk: English
The course aims at giving the student: - molecular-level understanding of the structure, stability, interactions and dynamics of proteins - knowledge about the principal physical methods used in modern protein science - practical experience in using some of these methods - the knowledge base needed to use and critically assess the protein research literature
Kunskap och förståelse
För godkänd kurs skall doktoranden
Färdighet och förmåga
För godkänd kurs skall doktoranden
Värderingsförmåga och förhållningssätt
För godkänd kurs skall doktoranden
- The chemical building-blocks and three-dimensional structures of proteins: Structure analysis by X-ray crystallography; Structure and sequence databases; Bioinformatics. - Protein characterization by optical spectroscopy: Physical principles and applications of fluorescence and circular dichroism spectroscopy. - Polypeptide conformation: Models of polymer conformation and conformational transitions; Conformational entropy; Folding cooperativity. - Protein energetics and stability: Packing; Hydration; Electrostatics; Thermal and solvent-induced denaturation; Differential scanning calorimetry. - Protein dynamics: Kinetic models; Proton exchange; Diffusion control; Protein folding; Computer simulation of proteins. - Nuclear magnetic resonance: Principles of NMR spectroscopy and relaxation; Analysis of structure, interactions and dynamics of proteins in solution. - Association processes: Ligand binding; Allostery; Protein aggregation; Isothermal titration calorimetry; Surface plasmon resonance.
The course literature consists of the compendium "Biophysical Chemistry", authored and updated each year by the course teachers, and of manuals for the practicals. For those who want go deeper, the compendium provides a list of selected references and links to the literature.
Undervisningsformer: Föreläsningar, seminarier, laborationer, projekt, studiebesök
Examinationsformer: Skriftlig tentamen, seminarieföredrag av deltagarna
Betygsskala: Underkänd, godkänd
Examinator:
Förutsatta förkunskaper: Under graduate courses in physical chemistry or physics including thermodynamics, intermolecular interactions and molecular dynamics.
Kursansvarig: Kristofer Modig <kristofer.modig@lth.lu.se>
Hemsida: http://www.cmps.lu.se/bpc/education/kfk032/