Valid from: Autumn 2016
Decided by: Rektor/Viktor Öwall
Date of establishment: 2016-08-30
Course type: Third-cycle course
Teaching languages: English, Swedish
The purpose of this course is to provide a scientific basis for cell culture work, both with regard to practical work and safety aspects. The goal is to provide insight and knowledge about general techniques in cell culturing as well as those techniques of particular relevance for the individual project. This includes e.g. furnishing and equipment of a cell culture laboratory, appropriate usage of devices, disinfection and sterilisation, laboratory safety and waste management, microbial contaminations, cross-contamination, media, cell adhesion, subcultivation, growth curves, freezing and thawing of cell culture and cloning/selection as general techniques. More specific ones may comprise for example establishing primary cell cultures, immortalisation, production of monoclonal antibodies, transfection, flow cytometry, and certain toxicity tests.
Knowledge and Understanding
For a passing grade the doctoral student must be able to present basic principles in cell culturing, furthermore rules around safety and waste management.
Competences and Skills
For a passing grade the doctoral student must
Judgement and Approach
For a passing grade the doctoral student must be able to reflect about cell culture work from a safety and environmental risk perspective.
In the course, safety aspects, the practical work in a cell culture lab as well as a theoretical background will be discussed.
Fundamental Techniques in Cell Culture, Laboratory Handbook (3rd edition, ECACC, Sigma-Aldrich) - whole book Appropriate additional for non-Swedish speakers will be chosen together with supervisor
Type of instruction: Self-study literature review
Examination format: Oral exam
Grading scale: Failed, pass
Admission requirements: Accepted as Phd student at LTH or expected to be accepted as such.
Assumed prior knowledge: Basic knowledge in cellbiology.