Gäller från och med: Autumn 2016
Beslutad av: Rektor/Viktor Öwall
Datum för fastställande: 2016-08-30
Avdelning: Immunotechnology
Kurstyp: Ren forskarutbildningskurs
Undervisningsspråk: English, Swedish
The purpose of this course is to provide a scientific basis for cell culture work, both with regard to practical work and safety aspects. The goal is to provide insight and knowledge about general techniques in cell culturing as well as those techniques of particular relevance for the individual project. This includes e.g. furnishing and equipment of a cell culture laboratory, appropriate usage of devices, disinfection and sterilisation, laboratory safety and waste management, microbial contaminations, cross-contamination, media, cell adhesion, subcultivation, growth curves, freezing and thawing of cell culture and cloning/selection as general techniques. More specific ones may comprise for example establishing primary cell cultures, immortalisation, production of monoclonal antibodies, transfection, flow cytometry, and certain toxicity tests.
Kunskap och förståelse
För godkänd kurs skall doktoranden be able to present basic principles in cell culturing, furthermore rules around safety and waste management.
Färdighet och förmåga
För godkänd kurs skall doktoranden
Värderingsförmåga och förhållningssätt
För godkänd kurs skall doktoranden be able to reflect about cell culture work from a safety and environmental risk perspective.
In the course, safety aspects, the practical work in a cell culture lab as well as a theoretical background will be discussed.
Fundamental Techniques in Cell Culture, Laboratory Handbook (3rd edition, ECACC, Sigma-Aldrich) - whole book Appropriate additional for non-Swedish speakers will be chosen together with supervisor
Undervisningsform: Litteraturkurs som självstudier
Examinationsform: Muntlig tentamen
Betygsskala: Underkänd, godkänd
Examinator:
Förkunskapskrav: Accepted as Phd student at LTH or expected to be accepted as such.
Förutsatta förkunskaper: Basic knowledge in cellbiology.
Kursansvariga: