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Third-Cycle Courses

Faculty of Engineering | Lund University

Details for Course KIM060F Protein Microarray Techniques

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General
  • KIM060F
  • Temporary
Course Name
  • Protein Microarray Techniques
Course Extent
  • 3
Type of Instruction
  • Third-cycle course
Administrative Information
  • 7717 (Immunotechnology)
  • 2016-11-11
  • Mats Ohlin

Current Established Course Syllabus

General
Aim
  • Protein microarrays represents a novel technology for multiplexed, high-throughput protein expression profiling of crude biological samples, such as blood, cell lysates and tissue extracts. In this one-week full-time hands-on course, you will learn about the theory and practical work behind state-of-the-art protein microarray technologies in proteomics.
Contents
  • The course will present the fundamentals behind designing, producing, running and analysing protein microarrays for protein expression profiling. Theories behind all the different steps are discussed and experiments (exercises) are performed. The principles behind generating numerous probes using combinatorial biology will be presented. A variety of array designs and formats, in both the micro- and nano-scale dimensions, will be covered. Present and future applications will be discussed. The practical work will be illustrated by producing, running and analysing antibody microarrays, including data handling and bioinformatics.
Knowledge and Understanding
  • For a passing grade the doctoral student must
  • describe the principles behind protein microarrays, including fundamental design of protein arrays and how it works.
    describe applications for protein microarrays, and discuss the possibilities and limitations of the technology.
Competences and Skills
  • For a passing grade the doctoral student must
  • design a protein microarray set-up (in theory).
    make an experimental plan for how to perform protein expression analysis of biological samples using protein microarrays.
    perform protein microarray analysis, and be able to process and analyze data thereof.
    explain the kinds of results that may be obtained from various protein microarrays setups.
Judgement and Approach
  • For a passing grade the doctoral student must
  • account for suitability / choice of technology for protein expression profiling.
    evaluate the differences in suitability for protein expression profiling compared to other techniques from an analytical / technical point of view.
Types of Instruction
  • Lectures
  • Laboratory exercises
  • Exercises
  • presentations and group discussions
Examination Formats
  • Miscellaneous
  • Attendance (> 95%) in lectures, practical work and exercises. Active participation at a level relevant for the learning objectives in practical work, discussions, exercises and presentation.
  • Failed, pass
Admission Requirements
  • Admitted to postgraduate education
Assumed Prior Knowledge
  • Fundamental knowledge in biorelated subjects
Selection Criteria
  • PhD students at Lund University have priority
Literature
  •  
  • Lecture hand-outs, articles, and practical lab-guides
Further Information
  • The course is given every autumn with the framework of the LifeScience courses (http://www.cmps.lu.se/life_sciences) provided that 6 to 8 students are accepted.
Course code
  • KIM060F
Administrative Information
  • 2016-11-11
  • Mats Ohlin

All Established Course Syllabi

1 course syllabus.

Valid from First hand in Second hand in Established
Autumn 2016 2016‑04‑25 10:59:08 2016‑09‑20 12:55:45 2016‑11‑11

Current or Upcoming Published Course Occasion

No matching course occasion was found.

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