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Protein microarrays represents a novel technology for multiplexed, high-throughput protein expression profiling of crude biological samples, such as blood, cell lysates and tissue extracts. In this one-week full-time hands-on course, you will learn about the theory and practical work behind state-of-the-art protein microarray technologies in proteomics.

The course will present the fundamentals behind designing, producing, running and analysing protein microarrays for protein expression profiling. Theories behind all the different steps are discussed and experiments (exercises) are performed. The principles behind generating numerous probes using combinatorial biology will be presented. A variety of array designs and formats, in both the micro- and nano-scale dimensions, will be covered. Present and future applications will be discussed. The practical work will be illustrated by producing, running and analysing antibody microarrays, including data handling and bioinformatics.

describe the principles behind protein microarrays, including fundamental design of protein arrays and how it works.
describe applications for protein microarrays, and discuss the possibilities and limitations of the technology.

design a protein microarray set-up (in theory).
make an experimental plan for how to perform protein expression analysis of biological samples using protein microarrays.
perform protein microarray analysis, and be able to process and analyze data thereof.
explain the kinds of results that may be obtained from various protein microarrays setups.

account for suitability / choice of technology for protein expression profiling.
evaluate the differences in suitability for protein expression profiling compared to other techniques from an analytical / technical point of view.

Föreläsningar

Laborationer

övningar

presentations and group discussions

övrigt

Attendance (> 95%) in lectures, practical work and exercises. Active participation at a level relevant for the learning objectives in practical work, discussions, exercises and presentation.

Underkänd, godkänd

Admitted to postgraduate education

Fundamental knowledge in biorelated subjects

PhD students at Lund University have priority

 

Lecture hand-outs, articles, and practical lab-guides

The course is given every autumn with the framework of the LifeScience courses (http://www.cmps.lu.se/life_sciences) provided that 6 to 8 students are accepted.

KIM060F

 -11-11

Mats Ohlin