Valid from: Autumn 2016
Decided by: Mats Ohlin
Date of establishment: 2016-11-11
Division: Immunotechnology
Course type: Third-cycle course
Teaching languages: English, Swedish
Protein microarrays represents a novel technology for multiplexed, high-throughput protein expression profiling of crude biological samples, such as blood, cell lysates and tissue extracts. In this one-week full-time hands-on course, you will learn about the theory and practical work behind state-of-the-art protein microarray technologies in proteomics.
Knowledge and Understanding
For a passing grade the doctoral student must
Competences and Skills
For a passing grade the doctoral student must
Judgement and Approach
For a passing grade the doctoral student must
The course will present the fundamentals behind designing, producing, running and analysing protein microarrays for protein expression profiling. Theories behind all the different steps are discussed and experiments (exercises) are performed. The principles behind generating numerous probes using combinatorial biology will be presented. A variety of array designs and formats, in both the micro- and nano-scale dimensions, will be covered. Present and future applications will be discussed. The practical work will be illustrated by producing, running and analysing antibody microarrays, including data handling and bioinformatics.
Lecture hand-outs, articles, and practical lab-guides
Types of instruction: Lectures, laboratory exercises, exercises. presentations and group discussions
Examination format: Miscellaneous.
Attendance (> 95%) in lectures, practical work and exercises. Active participation at a level relevant for the learning objectives in practical work, discussions, exercises and presentation.
Grading scale: Failed, pass
Examiner:
Admission requirements: Admitted to postgraduate education
Assumed prior knowledge: Fundamental knowledge in biorelated subjects
Selection criteria: PhD students at Lund University have priority
The course is given every autumn with the framework of the LifeScience courses (http://www.cmps.lu.se/life_sciences) provided that 6 to 8 students are accepted.
Course coordinators:
Web page: http://www.immun.lth.se/education/graduate-student-course/