Protein Microarray Techniques
Proteinmikromatristekniker

KIM060F, 3 credits

Valid from: Autumn 2016
Decided by: Mats Ohlin
Date of establishment: 2016-11-11

General Information

Division: Immunotechnology
Course type: Third-cycle course
Teaching languages: English, Swedish

Aim

Protein microarrays represents a novel technology for multiplexed, high-throughput protein expression profiling of crude biological samples, such as blood, cell lysates and tissue extracts. In this one-week full-time hands-on course, you will learn about the theory and practical work behind state-of-the-art protein microarray technologies in proteomics.

Goals

Knowledge and Understanding

For a passing grade the doctoral student must

• describe the principles behind protein microarrays, including fundamental design of protein arrays and how it works.
• describe applications for protein microarrays, and discuss the possibilities and limitations of the technology.

Competences and Skills

For a passing grade the doctoral student must

• design a protein microarray set-up (in theory).
• make an experimental plan for how to perform protein expression analysis of biological samples using protein microarrays.
• perform protein microarray analysis, and be able to process and analyze data thereof.
• explain the kinds of results that may be obtained from various protein microarrays setups.

Judgement and Approach

For a passing grade the doctoral student must

• account for suitability / choice of technology for protein expression profiling.
• evaluate the differences in suitability for protein expression profiling compared to other techniques from an analytical / technical point of view.

Course Contents

The course will present the fundamentals behind designing, producing, running and analysing protein microarrays for protein expression profiling. Theories behind all the different steps are discussed and experiments (exercises) are performed. The principles behind generating numerous probes using combinatorial biology will be presented. A variety of array designs and formats, in both the micro- and nano-scale dimensions, will be covered. Present and future applications will be discussed. The practical work will be illustrated by producing, running and analysing antibody microarrays, including data handling and bioinformatics.

Course Literature

Lecture hand-outs, articles, and practical lab-guides

Instruction Details

Types of instruction: Lectures, laboratory exercises, exercises. presentations and group discussions

Examination Details

Examination format: Miscellaneous. Attendance (> 95%) in lectures, practical work and exercises. Active participation at a level relevant for the learning objectives in practical work, discussions, exercises and presentation.
Grading scale: Failed, pass
Examiner:

Admission Details

Admission requirements: Admitted to postgraduate education
Assumed prior knowledge: Fundamental knowledge in biorelated subjects
Selection criteria: PhD students at Lund University have priority

Further Information

The course is given every autumn with the framework of the LifeScience courses (http://www.cmps.lu.se/life_sciences) provided that 6 to 8 students are accepted.

Course Occasion Information

Contact and Other Information

Course coordinators:
Web page: http://www.immun.lth.se/education/graduate-student-course/