English

Every autumn semester

The purpose of the course is to provide participants with practical experience and theoretical knowledge of flow cytometric analysis of microbial cell populations through wet and dry lab exercises.

The course consists of compulsory lectures, seminars, workshops, oral or written exercises, and wet experimental and dry projects carried out in groups of 1-4 people.

On completion of the course, participants shall be able to:
Comprehend principles of the flow cytometry instrument, including basic knowledge in the design of the instrument and its operation.
Have knowledge of the most common microbial flow cytometry applications, including possibilities and limitations for analysis and preparative sorting of microbial populations.
Analyse and interpret data obtained from flow cytometry.

On completion of the course, participants shall be able to:
Make an experimental plan for analyzing multiple user-specified fluorescence of cells, and use flow cytometry to analyse microbial populations, including setting up experiments and performing analysis of user-specified properties of microbial populations.

On completion of the course, participants shall be able to:
Explain type of results that can be obtained with flow cytometry.
Compare results from different analyses and from an analytical point of view understand similarities/differences.
Summarise flow cytometry results in a report

Lectures

Laboratory exercises

Exercises

Project

Self-study literature review

Written assignments

Seminars given by participants

Possible grades are Pass and Fail. To pass the course, the student must have (1) >95% attendance to all lectures and practical exercises, (2) handed in written reports and (3) made an oral presentation summarizing the literature study.

Failed, pass

Basic knowledge in microbiology.

Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/). Applications are received each year between May 1st and May 31st and all courses are given September-December. Please note that the courses are not open to master students! You have to certify that you are a registered PhD student when you apply.

 

Course literature is stated before course start.

KMB015F

2020-11-20

Senior lecturer Åsa Håkansson