Microbial Flow Cytometry
Mikrobiell flödescytometri

KMB020F, 3 credits

Valid from: Autumn 2020
Decided by: Senior lecturer Åsa Håkansson
Date of establishment: 2021-01-25

General Information

Division: Applied Microbiology
Course type: Third-cycle course
Teaching language: English

Aim

The purpose of the course is to provide participants with practical experience and theoretical knowledge of flow cytometric analysis of microbial cell populations

Goals

Knowledge and Understanding

For a passing grade the doctoral student must

• Be able to comprehend principles of the flow cytometry instrument, including basic knowledge in the design of the instrument and its operation.
• Be able to describe the most common microbial flow cytometry applications, including possibilities and limitations for analysis and preparative sorting of microbial populations.
• Be able to analyse and interpret data obtained from flow cytometry.

Competences and Skills

For a passing grade the doctoral student must

• Have the ability to make an experimental plan and perform the analysis of microbial populations with multiple-fluorescence flow cytometry.
• Be able to process and analyse of flow cyometry raw data with selected software.

Judgement and Approach

For a passing grade the doctoral student must

• Be able to explain type of results that can be obtained with flow cytometry.
• Be able to compare results from different analyses and from an analytical point of view understand similarities/differences.
• Be able to summarise flow cytometry results in a report.

Course Contents

The course consists of compulsory lectures, seminars, workshops, oral or written exercises, and experimental projects carried out in groups of 1-4 people.

Course Literature

Wilkinson, M.: Flow Cytometry in Microbiology. ISBN 9781910190111.

Instruction Details

Types of instruction: Lectures, laboratory exercises, exercises, project, self-study literature review

Examination Details

Examination formats: Written report, seminars given by participants, miscellaneous. To pass the course, the student must have (1) >95% attendance to all lectures and practical exercises, (2) handed in a written report and (3) made an oral presentation summarizing the literature study.
Grading scale: Failed, pass
Examiner:

Admission Details

Admission requirements: Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/). Applications are received each year between May 1st and May 31st.
Assumed prior knowledge: Basic knowledge in microbiology
Selection criteria: Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/).

Further Information

The number of course participants is max 8 and min 6. If too few students are registered the course is not given.

Course Occasion Information

Contact and Other Information

Course coordinator: Magnus Carlquist <magnus.carlquist@tmb.lth.se>