DNA Amplification Technology
DNA-amplifieringsteknik

KMB025F, 3 högskolepoäng

Gäller från och med: Autumn 2022
Beslutad av: Åsa Håkansson
Datum för fastställande: 2022-10-25

Allmänna uppgifter

Avdelning: Applied Microbiology
Kurstyp: Ren forskarutbildningskurs
Undervisningsspråk: English

Syfte

The aim of the course is to give participants practical experience and theoretical knowledge of PCR-based analysis of nucleic acids (RNA/DNA) through lectures, wet laboratory work and discussions.

Mål

Kunskap och förståelse

För godkänd kurs skall doktoranden

• Explain the biochemical and physical principles that govern polymerase chain reaction (PCR)
• Explain the concepts of detection limit and specificity linked to PCR-based analysis.
• Have knowledge of the principles for quality assurance of diagnostic DNA analysis
• Have knowledge of how different types of PCR inhibitors interfere with the analysis, and how to counteract these effects
• Have knowledge of how PCR is used in diagnostic analysis, including forensic DNA analysis

Färdighet och förmåga

För godkänd kurs skall doktoranden

• Perform quantitative PCR (absolute and relative quantification of nucleic acids)
• Design and optimize assays for conventional PCR and qPCR
• Determine which types of controls are needed to ensure the quality of a specific analysis process
• Evaluate and interpret results from conventional PCR and qPCR including troubleshooting

Värderingsförmåga och förhållningssätt

För godkänd kurs skall doktoranden

• Explain which PCR technique (conventional PCR, qPCR, RT-qPCR or dPCR) is best suited for a particular type of nucleic acid analysis
• Evaluate how reliable a DNA result is and explain what determines whether the result is reliable or not

Kursinnehåll

The course consists of compulsory lectures, seminars, workshops, literature assignments and wet labs that are performed individually or in groups of 2-3 people.

Kurslitteratur

The course literature consists of selected scientific articles which are handed to the participants when the course starts..

Kursens undervisningsformer

Undervisningsformer: Föreläsningar, seminarier, laborationer, övningar, projekt, litteraturkurs som självstudier

Kursens examination

Examinationsformer: Inlämningsuppgifter, seminarieföredrag av deltagarna
Betygsskala: Underkänd, godkänd
Examinator:

Antagningsuppgifter

Förkunskapskrav: Information about the application is available on the website http://www.cmps.lu.se/life-sciences/. Applications are accepted between 1 May and 31 May and the course is given for one week during September-December. Please note that the course is only available to registered doctoral students, which must be certified at the time of application.
Förutsatta förkunskaper: Basic knowledge in biochemistry
Urvalskriterier: Please note that the course is only available to registered doctoral students, which must be certified at the time of application. Students from Lund University normally have priority, but students from other higher education institutions are also warmly welcome to apply for a place

Kurstillfällesinformation

Kontaktinformation och övrigt

Kursansvarig: Johannes Hedman <johannes.hedman@ple.lth.se>
Hemsida: https://www.cmps.lu.se/life-sciences/courses/dna-amplification-technology-week-42/