Metabolic Engineering

KMBN05F, 7.5 högskolepoäng

Gäller från och med: Autumn 2021
Beslutad av: Åsa Håkansson
Datum för fastställande: 2022-04-04

Allmänna uppgifter

Avdelning: Applied Microbiology
Kurstyp: Gemensam kurs, avancerad nivå och forskarnivå
Kursen ges även på avancerad nivå med kurskoder: KMBN05, KMB040
Undervisningsspråk: English

Syfte

Based on previous student knowledge in gene technology, mathematics and microbiology, the course aims to introduce the PhD students to the field of metabolic engineering, discuss tools and strategies used in the field and give the students insight in how the technology can be used in industrial biotechnology.

Mål

Kunskap och förståelse

För godkänd kurs skall doktoranden

• - Understand the fundamentals of metabolic engineering tools (including mass balances, metabolic flux modeling, metabolic control analysis)
• - Understand how these tools can be applied for the analysis of metabolically engineered microorganisms
• - Have acquired in depth knowledge on metabolic pathways and their regulation and on how metabolic pathways can be engineered for the improvement of various bioprocesses.

Färdighet och förmåga

För godkänd kurs skall doktoranden

• - Show ability to carry out relevant calculations concerning mass balances, metabolic flux analysis, metabolic control analysis in biological systems
• - Show ability to analyse experimental work aiming at characterising genetically modified microorganisms and corresponding control organisms
• -Show ability to understand and master the terminology in the field in verbal and written form and to organise and structure the scientific content into a whole
• - Show ability to present a critical report of results from scientific papers on metabolic engineering

Värderingsförmåga och förhållningssätt

För godkänd kurs skall doktoranden

• - Show ability to do assessments of other student and researcher contributions in the field of metabolic engineering
• - Show ability to relate and distinguish between evidence and argument

Kursinnehåll

The course includes lectures on the following topics: strain engineering, advanced genetic tools, metabolic pathways, metabolic control analysis, metabolic flux analysis, omics and systems biology. The literature study consists of a thorough study of research strategies on a specific topic within metabolic engineering. In the lab simulation, the objective is to critically evaluate data arising from a set of experiments where a biofuel or bulk chemical is produced from sugars with wild-type and genetically engineered microorganisms. This includes the analysis of growth and product formation, enzyme activity and omics data,. Exercises are performed on carbon balance, redox balance, flux analysis and metabolic control analysis.

Kurslitteratur

Course compendium

Kursens undervisningsformer

Undervisningsformer: Föreläsningar, seminarier, laborationer, övningar, litteraturkurs som självstudier

Kursens examination

Examinationsformer: Skriftlig tentamen, muntlig tentamen, skriftlig rapport
Betygsskala: Underkänd, godkänd
Examinator:

Antagningsuppgifter

Förkunskapskrav: Application to the course responsible
Förutsatta förkunskaper: Microbiology, Gene Technology

Kurstillfällesinformation

Kontaktinformation och övrigt

Kursansvariga:
Hemsida: https://canvas.education.lu.se/