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Third-Cycle Courses

Faculty of Engineering | Lund University

Details for the Course Syllabus for Course FBRN10F valid from Spring 2021

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General
Aim
  • The course aims to provide knowledge on light interactions with diverse biological tissue found in living beings, organic matter and our natural surroundings. Understanding these interactions allows to select appropriate techniques and design optimal instrumentation for probing key features to characterize, classify or grade the health/quality of diverse biological samples. The course grasps spatial scales from microscopic cellular level, through the macroscopic regime to remote sensing of our biosphere. The course covers underlying
    physical principles of light in biology with a tour of biophotonic instrumental approaches developed until today. For deepened understanding and practical experience the course will offer a creative hands-on project for student groups to freely develop a simple setup for a biophotonic technique, apply it to a selected sample and present it at the end of the course.The course equips the student with a unique engineering tool-set valuable for development and application of modern photonics in life sciences.
Contents
  • The course in biophotonics provides an introduction to optical phenomena encountered when measuring on biological samples and introduces optical diagnostic methods and instrumentation employed for evaluating the state of biological tissue and organic matter. Methods include: Remote sensing techniques for studies of biological flora and fauna. Practical laser spectroscopy based on light emitting diodes for measurements of absorption and fluorescence. Polarization techniques for analysis of light scattered from biological samples. Furthermore, the course includes imaging and microscopy techniques for investigations of small structures. Moreover, aspects of data analysis are treated. A particular emphasis is put on projects letting student groups build their own instrumentation for a variety of case studies. The projects will be oriented around the combination of an instrumental approach with a biological sample and will include aspects of optoelectronics, optomechanical construction, acquisition, measurements, data interpretation and presentation. Two other laboratory exercises deal with polarization of light scattered from biological samples, spectral imaging and microscopy. Additional insights in biophotonic diagnostics are gained from visits to laboratories of research partners at Lund University and related local industry.
Knowledge and Understanding
  • For a passing grade the doctoral student must
  • - be able to explain the principles of light interaction with the types of biological samples treated in the course.
    - be able to explain the principles of the diagnostic techniques treated in the course.
    - be able to understand the concepts of data analysis treated in the course.
Competences and Skills
  • For a passing grade the doctoral student must
  • - be able to select and combine methods and instrumentation suitable for a biophotonic application.
    - be able to plan and carry out an experimental project on combination and utilization of biophotonic instrumentation.
    - be able to orally present results from an experimental study.
    - be able to write reports from laboratory exercises with analysis and discussion of results.
Judgement and Approach
  • For a passing grade the doctoral student must
  • - be able to understand possibilities and limitations for the instrumentation and measurement techniques.
    - be able to find and assess information from sources outside the course material.
    - be able to assimilate information from scientific papers and advanced textbooks.
Types of Instruction
  • Lectures
  • Laboratory exercises
  • Exercises
  • Project
  • Study visit
  • The exercise is intended for preparation of the course project.
Examination Formats
  • Written exam
  • Seminars given by participants
  • To pass the course as a whole requires pass on laboratory exercises and the written exam. The laboratory exercises are carried out in groups of two, with an oral presentation for the workshop project and written reports for the two other exercises. For the exam,
    understanding of the course contents is more important than memorizing. The final grade is dictated by the result achieved on the written exam.
  • Failed, pass
Admission Requirements
Assumed Prior Knowledge
  • Basic courses in physics and optics, FAFN01 Lasers, FAFN25 Atomic and Molecular Spectroscopy.
Selection Criteria
Literature
  • Boudoux, C.: Fundamentals of Biomedical Optics. 2017. ISBN 9781366451194.
    Hobbs, Philip C. D.: Building Electro-Optical Systems: Making It all Work. Wiley, 2009. ISBN 9780470402290.
Further Information
Course code
  • FBRN10F
Administrative Information
  • 2021-03-26
  • Anders Gsutafsson / FUN

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