English

Varje hösttermin

The aim of the course is to provide advanced knowledge in and insight into analytical separation techniques.

The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), and FFF (flow field fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.
An important part of the course is spent to strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

be able to explain advanced theories in different analytical principles and techniques comprised by the course
be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low molecular weight compounds
be able to understand the importance of adequate sample preparation prior to instrumental analysis
be able to explain the meaning of concepts used in quality assessment of analytical results

will be able to modify/optimize different separations methods both for quantitative and qualitative purposes
will be able to evaluate the choice of separation method for the analysis of low molecular weight substances, based on the characteristics of the substances and the sample

understand how the choice of an analytical technique and method affects the quality of the analytical result.

Föreläsningar

Seminarier

övningar

Litteraturkurs som självstudier

Skriftlig tentamen

Seminarieföredrag av deltagarna

Underkänd, godkänd

KAKF01 Analytisk kemi 9 hp or KEMB06 Analytisk kemi 15 hp or equivalent knowledge that has been gained in another way

F., P.: The essence of chromatography. Elsevier, 2003. ISBN 9780444501981.

The book is available as e-book:
http://libguides.lub.lu.se/content.php?pid=288754&sid=4241646

KAK050F

 -05-13

FN2/Eva Nordberg Karlsson