Flow cytometry is widely used for analysis of immune cell characteristics as well as for sorting of pure cell populations.
This is a basic course in flow cytometry, which covers various applications of the technique, including analysis of extracellular and intracellular proteins, cell viability, cell proliferation, detection of proteins in solution and cell sorting. We will discuss important factors including compensation, selection and combination of fluorochromes and selection of appropriate control samples. The course also comprise a seminar to give basic understanding of how a flow cytometer instrument works.
Practical parts will be performed on human immune cells in our cell lab, and samples will be analysed on BD FACS Canto and BD FACS Aria II instruments.