Chromatographic Analysis
Kromatografisk analys

KAK050F, 7.5 credits

Valid from: Autumn 2013
Decided by: FN2/Eva Nordberg Karlsson
Date of establishment: 2014-05-13

General Information

Division: Centre for Analysis and Synthesis (CAS)
Course type: Course given jointly for second and third cycle
The course is also given at second-cycle level with course codes: KASN05, KEMM76, KAK050
Teaching language: English

Aim

The aim of the course is to provide advanced knowledge in and insight into analytical separation techniques.

Goals

Knowledge and Understanding

For a passing grade the doctoral student must

• be able to explain advanced theories in different analytical principles and techniques comprised by the course
• be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low molecular weight compounds
• be able to understand the importance of adequate sample preparation prior to instrumental analysis
• be able to explain the meaning of concepts used in quality assessment of analytical results

Competences and Skills

For a passing grade the doctoral student must

• will be able to modify/optimize different separations methods both for quantitative and qualitative purposes
• will be able to evaluate the choice of separation method for the analysis of low molecular weight substances, based on the characteristics of the substances and the sample

Judgement and Approach

For a passing grade the doctoral student must understand how the choice of an analytical technique and method affects the quality of the analytical result.

Course Contents

The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), and FFF (flow field fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis. An important part of the course is spent to strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

Course Literature

F., P.: The essence of chromatography. Elsevier, 2003. ISBN 9780444501981.
The book is available as e-book: http://libguides.lub.lu.se/content.php?pid=288754&sid=4241646

Instruction Details

Types of instruction: Lectures, seminars, exercises, self-study literature review

Examination Details

Examination formats: Written exam, seminars given by participants
Grading scale: Failed, pass
Examiner:

Admission Details

Admission requirements: KAKF01 Analytisk kemi 9 hp or KEMB06 Analytisk kemi 15 hp or equivalent knowledge that has been gained in another way

Course Occasion Information

Contact and Other Information

Course coordinator: Margareta Sandahl <margareta.sandahl@chem.lu.se>