Cell Culture Management and Safety in Mammalian Cell Culture Work
Cellodling - praktiskt handhavande och säkerhet i arbete med mammalieceller

KIM050F, 1.5 credits

Valid from: Autumn 2016
Decided by: Rektor/Viktor Öwall
Date of establishment: 2016-08-30

General Information

Division: Immunotechnology
Course type: Third-cycle course
Teaching languages: English, Swedish

Aim

The purpose of this course is to provide a scientific basis for cell culture work, both with regard to practical work and safety aspects. The goal is to provide insight and knowledge about general techniques in cell culturing as well as those techniques of particular relevance for the individual project. This includes e.g. furnishing and equipment of a cell culture laboratory, appropriate usage of devices, disinfection and sterilisation, laboratory safety and waste management, microbial contaminations, cross-contamination, media, cell adhesion, subcultivation, growth curves, freezing and thawing of cell culture and cloning/selection as general techniques. More specific ones may comprise for example establishing primary cell cultures, immortalisation, production of monoclonal antibodies, transfection, flow cytometry, and certain toxicity tests.

Goals

Knowledge and Understanding

For a passing grade the doctoral student must be able to present basic principles in cell culturing, furthermore rules around safety and waste management.

Competences and Skills

For a passing grade the doctoral student must

• show insight and overview with regard to different aspects of cell culture work and the student should be able to explain and discuss cell culture routines and rules in a scientific way.
• be able to reflect and argument with regard to techniques and routines used in a cell culture lab.
• able to choose appropriate ways to handle material in a cell lab.

Judgement and Approach

For a passing grade the doctoral student must be able to reflect about cell culture work from a safety and environmental risk perspective.

Course Contents

In the course, safety aspects, the practical work in a cell culture lab as well as a theoretical background will be discussed.

Course Literature

Fundamental Techniques in Cell Culture, Laboratory Handbook (3rd edition, ECACC, Sigma-Aldrich) - whole book Appropriate additional for non-Swedish speakers will be chosen together with supervisor

Instruction Details

Type of instruction: Self-study literature review

Examination Details

Examination format: Oral exam
Grading scale: Failed, pass
Examiner:

Admission Details

Admission requirements: Accepted as Phd student at LTH or expected to be accepted as such.
Assumed prior knowledge: Basic knowledge in cellbiology.

Course Occasion Information

Contact and Other Information

Course coordinators: