Microbial Flow Cytometry
Mikrobiell flödescytometri

KMB015F, 3 credits

Valid from: Autumn 2019
Decided by: Senior lecturer Åsa Håkansson
Date of establishment: 2020-11-20

General Information

Division: Applied Microbiology
Course type: Third-cycle course
Teaching language: English

Aim

The purpose of the course is to provide participants with practical experience and theoretical knowledge of flow cytometric analysis of microbial cell populations through wet and dry lab exercises.

Goals

Knowledge and Understanding

For a passing grade the doctoral student must

• On completion of the course, participants shall be able to:
• Comprehend principles of the flow cytometry instrument, including basic knowledge in the design of the instrument and its operation.
• Have knowledge of the most common microbial flow cytometry applications, including possibilities and limitations for analysis and preparative sorting of microbial populations.
• Analyse and interpret data obtained from flow cytometry.

Competences and Skills

For a passing grade the doctoral student must

• On completion of the course, participants shall be able to:
• Make an experimental plan for analyzing multiple user-specified fluorescence of cells, and use flow cytometry to analyse microbial populations, including setting up experiments and performing analysis of user-specified properties of microbial populations.

Judgement and Approach

For a passing grade the doctoral student must

• On completion of the course, participants shall be able to:
• Explain type of results that can be obtained with flow cytometry.
• Compare results from different analyses and from an analytical point of view understand similarities/differences.
• Summarise flow cytometry results in a report

Course Contents

The course consists of compulsory lectures, seminars, workshops, oral or written exercises, and wet experimental and dry projects carried out in groups of 1-4 people.

Course Literature

Course literature is stated before course start.

Instruction Details

Types of instruction: Lectures, laboratory exercises, exercises, project, self-study literature review

Examination Details

Examination formats: Written assignments, seminars given by participants. Possible grades are Pass and Fail. To pass the course, the student must have (1) >95% attendance to all lectures and practical exercises, (2) handed in written reports and (3) made an oral presentation summarizing the literature study.
Grading scale: Failed, pass
Examiner:

Admission Details

Assumed prior knowledge: Basic knowledge in microbiology.
Selection criteria: Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/). Applications are received each year between May 1st and May 31st and all courses are given September-December. Please note that the courses are not open to master students! You have to certify that you are a registered PhD student when you apply.

Course Occasion Information

Contact and Other Information

Course coordinators: