Microbial Flow Cytometry
Mikrobiell flödescytometri

KMB020F, 3 högskolepoäng

Gäller från och med: Autumn 2020
Beslutad av: Senior lecturer Åsa Håkansson
Datum för fastställande: 2021-01-25

Allmänna uppgifter

Avdelning: Applied Microbiology
Kurstyp: Ren forskarutbildningskurs
Undervisningsspråk: English

Syfte

The purpose of the course is to provide participants with practical experience and theoretical knowledge of flow cytometric analysis of microbial cell populations

Mål

Kunskap och förståelse

För godkänd kurs skall doktoranden

• Be able to comprehend principles of the flow cytometry instrument, including basic knowledge in the design of the instrument and its operation.
• Be able to describe the most common microbial flow cytometry applications, including possibilities and limitations for analysis and preparative sorting of microbial populations.
• Be able to analyse and interpret data obtained from flow cytometry.

Färdighet och förmåga

För godkänd kurs skall doktoranden

• Have the ability to make an experimental plan and perform the analysis of microbial populations with multiple-fluorescence flow cytometry.
• Be able to process and analyse of flow cyometry raw data with selected software.

Värderingsförmåga och förhållningssätt

För godkänd kurs skall doktoranden

• Be able to explain type of results that can be obtained with flow cytometry.
• Be able to compare results from different analyses and from an analytical point of view understand similarities/differences.
• Be able to summarise flow cytometry results in a report.

Kursinnehåll

The course consists of compulsory lectures, seminars, workshops, oral or written exercises, and experimental projects carried out in groups of 1-4 people.

Kurslitteratur

Wilkinson, M.: Flow Cytometry in Microbiology. ISBN 9781910190111.

Kursens undervisningsformer

Undervisningsformer: Föreläsningar, laborationer, övningar, projekt, litteraturkurs som självstudier

Kursens examination

Examinationsformer: Skriftlig rapport, seminarieföredrag av deltagarna, övrigt. To pass the course, the student must have (1) >95% attendance to all lectures and practical exercises, (2) handed in a written report and (3) made an oral presentation summarizing the literature study.
Betygsskala: Underkänd, godkänd
Examinator:

Antagningsuppgifter

Förkunskapskrav: Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/). Applications are received each year between May 1st and May 31st.
Förutsatta förkunskaper: Basic knowledge in microbiology
Urvalskriterier: Information on how to apply for the course can be found here (http://www.cmps.lu.se/life-sciences/).

Övrig information

The number of course participants is max 8 and min 6. If too few students are registered the course is not given.

Kurstillfällesinformation

Kontaktinformation och övrigt

Kursansvarig: Magnus Carlquist <magnus.carlquist@ple.lth.se>